凍結牛体外受精由来胚の生存性に及ぼす融解速度の影響

DOI Web Site Web Site 参考文献17件

書誌事項

タイトル別名
  • Effect of Thawing Rate on The Viability of Frozen Bovine Embryos Derived from In Vitro Fertilization
  • トウケツ ウシ タイガイ ジュセイ ユライ ハイ ノ セイゾンセイ ニ オヨボ

この論文をさがす

説明

The relationship between thawing rate and the viability of frozen bovine blastocysts derived from in vitro fertilization and culture was examined. Embryos were frozen in straws with 1.4M glycerol+0.2M sucrose(GS), 1.6M 1,2-propanediol(PG) or 1.8M ethylene glycol(EG). They were thawed at various rates by placing the straws in 10, 20, 30, 40 or 50 C water bath. As soon as the crystallized medium in the straw melted, the contents were drained into a petri dish. The embryos were transferred into culture medium and co-cultured with cumulus cells for up to 72 h. When embryos were frozen in GS, there were no significant differences in the proportion of survived embryos which recovered the blastocoel among the various thawing rates, but hatching rate of the embryos increased with increasing the thawing rate. When embryos were frozen in PG, the survival (94%) and hatching rates (77%) of embryos thawed in 20 C water bath (876 C/min) were significantly higher (P<0.05) than those observed in other thawing rates. When embryos were frozen in EG, there were no significant differences in the rates of survival (82∼94%) and development (73∼91%) with all the thawing rates.<br>

収録刊行物

参考文献 (17)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ