Insulin-Like Growth Factor-I Stimulated DNA Replication in Mouse Endometrial Stromal Cells
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- INOUE Ayako
- Department of Biology, Faculty of Science, Okayama University
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- TAKEUCHI Sakae
- Department of Biology, Faculty of Science, Okayama University
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- TAKAHASHI Sumio
- Department of Biology, Faculty of Science, Okayama University
Bibliographic Information
- Other Title
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- Insulin-Like Growth Factor-1 Stimulated DNA Replication in Mouse Endometrial Stromal Cells
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Abstract
Much evidence has suggested that sex steroid hormone-induced growth of uterine cells is mediated by polypeptide growth factors synthesized in uterine tissues. The present study aimed to clarify the effect of insulin-like growth factor-I (IGF-I) on the proliferation of mouse endometrial stromal cells obtained from immature mice. IGF-I and IGF-I receptor (type I) mRNAs were detected in the endometrial stromal cells. IGF-I increased bromodeoxyuridine (BrdU) uptake in the endometrial stromal cells, indicating an increase in DNA replication. E2 increased IGF-I mRNA levels in the endometrial stromal cells. IGF-I receptor is a tyrosine kinase receptor, and treatment with genistein, a tyrosine kinase inhibitor, reduced IGF-I-induced BrdU-uptake in the endometrial stromal cells. IGF-I signaling pathways involve mitogen-activated protein (MAP) kinase and phosphatidylinositol-3 kinase (PI-3 kinase). Treatment with 10-7 M of the MAP kinase inhibitor PD098059 and 10-5 M of the PI-3 kinase inhibitor LY294002 decreased IGF-I-induced BrdU-uptake in the endometrial stromal cells. However, LY294002 (10-5 M) also decreased the BrdU-uptake in the absence of IGF-I treatment. These results suggest that endometrial IGF-I is involved in the proliferation of endometrial stromal cells in a paracrine or autocrine manner, and that the MAP kinase pathway is involved in DNA replication of endometrial stromal cells.<br>
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 51 (3), 305-313, 2005
The Society for Reproduction and Development
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Keywords
Details 詳細情報について
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- CRID
- 1390001206335813504
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- NII Article ID
- 130000054719
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- NII Book ID
- AA10936678
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- COI
- 1:CAS:528:DC%2BD2MXhtVeku7fO
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- ISSN
- 13484400
- 09168818
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- NDL BIB ID
- 7345548
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- PubMed
- 15724079
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed