Role of nitric oxide in the regulation of superoxide dismutase and prostaglandin F2α production in bovine luteal endothelial cells
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- LEE Seunghyung
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- ACOSTA Tomas J.
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- NAKAGAWA Yuji
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
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- OKUDA Kiyoshi
- Laboratory of Reproductive Endocrinology, Graduate School of Natural Science and Technology, Okayama University
書誌事項
- タイトル別名
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- Role of Nitric Oxide in the Regulation of Superoxide Dismutase and Prostaglandin F2.ALPHA. Production in Bovine Luteal Endothelial Cells
- Role of nitric oxide in the regulation of superoxide dismutase and prostaglandin F2 a production in bovine luteal endothelial cells
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抄録
Prostaglandin F2α (PGF) induces a rapid reduction in progesterone production (functional luteolysis) followed by tissue degeneration and cell death (structural luteolysis). Reactive oxygen species (ROS) including nitric oxide (NO) play crucial roles in the luteolytic action of PGF. The local concentration of intraluteal ROS is controlled by superoxide dismutase (SOD), the main enzyme involved in the control of intraluteal ROS. To clarify the roles of NO in the regulation of SOD in luteolysis, we examined the effects of NO on SOD expression and activity in cultured bovine luteal endothelial cells (LECs) during short-term (2 h, mimicking functional luteolysis) and long-term (24 h, mimicking structural luteolysis) incubation. We also investigated whether NO modulates PGF production by LECs. LECs were isolated from mid-luteal phase CLs, and exposed to NONOate (a NO donor) for 2 or 24 h. SOD mRNA expression was stimulated by NONOate (10-100 μM) at 2 h (P<0.05). Moreover, 10 μM NONOate stimulated SOD protein expression and SOD activity at 2 h (P<0.05), whereas NONOate inhibited SOD mRNA and protein expressions at 24 h (P<0.05). NONOate stimulated PGF biosynthesis at both incubation times. The overall findings suggest that NO differently regulates SOD in cultured LECs, depending on the exposure time. Acute elevation of SOD may represent a response of LECs to protect themselves against oxidative stress induced by PGF during functional luteolysis, whereas a later reduction of SOD levels by NO may facilitate an excess of intraluteal ROS during structural luteolysis.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 56 (4), 454-459, 2010
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206335923968
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- NII論文ID
- 130000256779
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 10788865
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可