Effect of NGF on the Motility and Acrosome Reaction of Golden Hamster Spermatozoa In Vitro
-
- JIN WanZhu
- Laboratory of Veterinary Physiology, Graduate School, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University Department of Cellular Molecular Physiology, Joslin Diabetes Center & Harvard Medical School
-
- TANAKA Akane
- Laboratory of Veterinary Molecular Pathology and Therapeutics, Graduate School, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology
-
- WATANABE Gen
- Laboratory of Veterinary Physiology, Graduate School, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology Division of Animal Life Science, Graduate School, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology Laboratory of Veterinary Molecular Pathology and Therapeutics, Graduate School, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology
-
- MATSUDA Hiroshi
- Laboratory of Veterinary Molecular Pathology and Therapeutics, Graduate School, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology
-
- TAYA Kazuyoshi
- Laboratory of Veterinary Physiology, Graduate School, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology Division of Animal Life Science, Graduate School, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology Laboratory of Veterinary Molecular Pathology and Therapeutics, Graduate School, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology
この論文をさがす
抄録
Motility and fertilizing ability are known to be two important physiological attributes of a mature sperm, yet the mechanism by which spermatozoa mature and become motile remains largely unknown. It has been shown that nerve growth factor (NGF) is a protein essential for the development, maintenance and survival of the peripheral and central nervous systems. However, the presence of high levels of NGF protein and mRNA do not correlate with the innervations by NGF sensitive fibers in tissues such as the testis, prostate and seminal vesicles. These observations have shifted the attention of research to the role of NGF outside of the nervous system. Here, we demonstrate that NGF and its receptors TrkA and p75 are widely expressed in the testis, accessory reproductive organ, and the epididymal sperms. We also show that NGF stimulates two important aspects of sperm functions, motility and the acrosome reaction, in a time- and dose-dependent manner. NGF activated the sperm cell acrosome reaction, while addition of inhibitors specific for MAPK kinase significantly blocked the sperm acrosome reaction. Taken together, our findings suggest that NGF plays an integral role in sperm motility and the acrosome reaction through, at least in part, the MAPK signalling pathway.<br>
収録刊行物
-
- Journal of Reproduction and Development
-
Journal of Reproduction and Development 56 (4), 437-443, 2010
公益社団法人 日本繁殖生物学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001206335925632
-
- NII論文ID
- 10026621774
-
- NII書誌ID
- AA10936678
-
- ISSN
- 13484400
- 09168818
-
- NDL書誌ID
- 10788828
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可