Analysis of single nucleotide polymorphisms in the 3′ region of estrogen receptor 1 gene in normal and cryptorchid Miniature Dachshunds and Chihuahuas
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- PATHIRANA Indunil Nishantha
- Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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- TANAKA Kakeru
- Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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- KAWATE Noritoshi
- Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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- TSUJI Makoto
- Ishizuka Animal Hospital
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- KIDA Kayoko
- Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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- HATOYA Shingo
- Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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- INABA Toshio
- Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
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- TAMADA Hiromichi
- Department of Advanced Pathobiology, Graduate School of Life and Environmental Sciences, Osaka Prefecture University
書誌事項
- タイトル別名
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- Analysis of Single Nucleotide Polymorphisms in the 3' Region of the Estrogen Receptor 1 Gene in Normal and Cryptorchid Miniature Dachshunds and Chihuahuas
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This study was performed to examine the distribution of single nucleotide polymorphisms (SNPs) and estimated haplotypes in the canine estrogen receptor (ER) α gene (ESR1) and the association of them with different phenotypes of cryptorchidism (CO) in Miniature Dachshunds and Chihuahuas. Forty CO and 68 normal dogs were used, and CO was classified into unilateral (UCO; n=33) and bilateral CO (BCO; n=5) or into abdominal (ACO; n=16) and inguinal CO (ICO; n=22). Thirteen DNA fragments located in the 70-kb region at the 3' end of ESR1 were amplified by PCR and sequenced to examine 13 SNPs (#1-#13) reported in a canine SNP database. Ten SNPs (#1-#4, #7, #8, #10-#13) were not polymorphic, and 5 new SNPs (#14-#18) were discovered. A common haplotype block in normal, CO and CO phenotypes was identified for an approximately 20-kb region encompassing 4 SNPs (#14-#17). Allele, genotype and haplotype frequencies in CO without classification by phenotype and also in UCO, ACO and ICO phenotypes were not statistically different from the normal group. Significant differences in genotype frequencies and homozygosity for the estimated GTTG haplotype within the block were observed in BCO compared with the normal group, although the number of BCO animals was small. Our results demonstrate that the examined SNPs and haplotypes in the 3' end of canine ESR1 are not associated with unilateral, abdominal and inguinal CO phenotypes and CO per se in Miniature Dachshunds and Chihuahuas. Further studies are necessary to suggest a clear association between the ESR1 SNPs and bilateral CO in dogs.<br>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 56 (4), 405-410, 2010
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206335967104
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- NII論文ID
- 10026621610
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 10788768
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可