Milrinone Treatment of Bovine Oocytes During In Vitro Maturation Benefits Production of Nuclear Transfer Embryos by Improving Enucleation Rate and Developmental Competence

DOI Web Site Web Site PubMed 被引用文献1件 参考文献63件
  • NARUSE Kenji
    NARO Tohoku Agricultural Research Center, Iwate 020-0198, Japan
  • IGA Kosuke
    NARO Tohoku Agricultural Research Center, Iwate 020-0198, Japan
  • SHIMIZU Manabu
    NARO Tohoku Agricultural Research Center, Iwate 020-0198, Japan
  • TAKENOUCHI Naoki
    NARO Tohoku Agricultural Research Center, Iwate 020-0198, Japan
  • AKAGI Satoshi
    NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan
  • SOMFAI Tamas
    NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan
  • HIRAO Yuji
    NARO Tohoku Agricultural Research Center, Iwate 020-0198, Japan Present: NARO Institute of Livestock and Grassland Science, Ibaraki 305-0901, Japan

書誌事項

タイトル別名
  • Milrinone Treatment of Bovine Oocytes During <i>In Vitro</i> Maturation Benefits Production of Nuclear Transfer Embryos by Improving Enucleation Rate and Developmental Competence

この論文をさがす

抄録

In the production of cattle nuclear transfer embryos, the production efficiency is affected by the oocyte developmental competence and successful enucleation rate. This study investigated the effect of treating oocytes with milrinone, a phosphodiesterase inhibitor, on these two characteristics. When cumulus–oocyte complexes (COCs) were cultured for 19 h with 0, 50 or 100 μM of milrinone, the enucleation rate was significantly improved by 100 μM milrinone. However, milrinone treatment during in vitro maturation (IVM) also delayed meiotic progression by at least 2 h, which would affect the examination of enucleation rate and developmental competence of oocytes. Thus, in the second experiment, meiotic resumption was temporarily inhibited with butyrolactone I (BL-I; 100 μM, 18 h) to decrease the delayed maturation caused by milrinone; this enabled a more accurate comparison of the effects of milrinone after oocyte maturation. In nuclear transfer embryo production, oocytes treated with milrinone (100 μM, 20 h) showed a significantly higher rate of enucleation compared with that of control oocytes. This improved enucleation rate was associated with a closer location of the metaphase plate to the first polar body in the treated oocytes compared with that in control oocytes. Furthermore, milrinone improved the frequency of development to the blastocyst stage in the resulting embryos. In conclusion, milrinone supplementation during IVM improved enucleation rates by rendering the metaphase plate in close proximity to the first polar body, and this treatment also improved oocyte developmental competence. These benefits additively improved the yield of cloned embryos that developed to the blastocyst stage.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (63)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ