Sperm Penetration Assay as an Indicator of Bull Fertility
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- PARK Yoo-Jin
- Department of Animal Science & Technology and BET Research Institute, School of Bioresource & Bioscience, Chung-Ang University, Gyeonggi-Do 456-756, Korea
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- MOHAMED El-Sayed A.
- Department of Animal Science & Technology and BET Research Institute, School of Bioresource & Bioscience, Chung-Ang University, Gyeonggi-Do 456-756, Korea
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- OH Shin-Ae
- Department of Animal Science & Technology and BET Research Institute, School of Bioresource & Bioscience, Chung-Ang University, Gyeonggi-Do 456-756, Korea
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- YOON Sung-Jae
- Department of Animal Science & Technology and BET Research Institute, School of Bioresource & Bioscience, Chung-Ang University, Gyeonggi-Do 456-756, Korea
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- KWON Woo-Sung
- Department of Animal Science & Technology and BET Research Institute, School of Bioresource & Bioscience, Chung-Ang University, Gyeonggi-Do 456-756, Korea
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- KIM Heung-Ruil
- Dairy Cattle Improvement Center, National Agricultural Co-operative Federation, Gyeonggi-Do 412-030, Korea
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- LEE Myeung-Sik
- National Institute of Animal Science, Rural Development Association, Kangwon-Do 232-950, Korea
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- LEE Kichoon
- Department of Animal Sciences, The Ohio State University, OH 43210, USA
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- PANG Myung-Geol
- Department of Animal Science & Technology and BET Research Institute, School of Bioresource & Bioscience, Chung-Ang University, Gyeonggi-Do 456-756, Korea
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Description
To predict the fertility of frozen-thawed bull spermatozoa, a sperm penetration assay (SPA) using zona-free hamster oocytes was optimized, and the assay results were compared with data from field fertility expressed as the non-return rate (NRR). To increase sperm penetration, the spermatozoa were pre-incubated and coincubated with oocytes in media containing various concentrations of heparin (0 to 50 μg/ml). Coincubation with 10 μg/ml heparin showed the highest sperm penetration (P<0.05); it is considered to be the optimized SPA method. Sperm fertility index values obtained from WSPA were significantly correlated with the historic average NRR of 46 bulls (P<0.01). To determine the normal range for SPA, we established the lower limits of the sperm fertility index and set the cut-off value at 2.55, at which point the NRR was more than 70%, using the receiver operating characteristic curve. The overall accuracy for the 46 bulls was 95.7% (44/46) for both the low and high NRR, with a sensitivity of 95.5% (21/22) and a specificity of 95.8%. This protocol would make it easier to discriminate bulls according to their sperm fertilizing ability.
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 58 (4), 461-466, 2012
The Society for Reproduction and Development
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Details 詳細情報について
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- CRID
- 1390001206336090112
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- NII Article ID
- 10031067093
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- NII Book ID
- AA10936678
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- COI
- 1:STN:280:DC%2BC38rmvFSgug%3D%3D
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- ISSN
- 13484400
- 09168818
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- NDL BIB ID
- 023891845
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- PubMed
- 22522227
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed