卵胞退行における顆粒層細胞アポトーシスの制御

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  • 眞鍋 昇
    京都大学大学院農学研究科応用生物科学専攻生体機構学
  • 妙本 陽
    京都大学大学院農学研究科応用生物科学専攻生体機構学
  • 木村 嘉博
    京都大学大学院農学研究科応用生物科学専攻生体機構学
  • 今居 譲
    京都大学大学院農学研究科応用生物科学専攻生体機構学
  • 杉本 実紀
    京都大学大学院農学研究科応用生物科学専攻生体機構学
  • 宮本 元
    京都大学大学院農学研究科応用生物科学専攻生体機構学
  • 酒巻 和弘
    京都大学ウイルス研究所がんウイルス研究部門生体発がん機構学
  • 岡村 佳則
    京都大学大学院医学研究科第一病理学
  • 福本 学
    京都大学大学院医学研究科第一病理学

書誌事項

タイトル別名
  • Signal Transmission of Granulosa Cell Apoptosis in the Atretic Antral Follicles in the Pig Ovaries
  • ランポウ タイコウ ニ オケル カリュウソウ サイボウ アポトーシス ノ セイ

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抄録

The porcine antral follicles, 4-6 mm in diameter, were dissected from the ovaries of mature pigs, and then granulosa and cumulus cells were isolated from each follicle. In atretic follicles, apoptotic cells were observed histochemically among granulosa cells only. In atretic follicles, high activity of neutral Ca2+/Mg2+-dependent endonuclease by which the chromatin DNA is degraded at internucleosomal sites in the cells undergoing apoptosis was noted only in granulosa cells but not in cumulus cells. Low activity of the Ca2+/Mg2+-dependent endonuclease was observed in both types of cells obtained from healthy follicles. Low activities of neutral Ca2+-dependent endonuclease, neutral Mg2+-dependent endonuclease and acidic cation-independent endonuclease were demonstrated in all cases. A good correlation between the Ca2+/Mg2+-dependent endonuclease activity of granulosa cells and the progesterone/estradiol ratio of follicular fluid in each follicle, which provides an index of follicular atresia in pig ovaries, was found. In vitro study showed that the specific inhibitors for interleukin-β converting enzyme (ICE) like cysteine proteases could stop the apoptotic process induced by specific monoclonal antibody to granulosa cell membrane protein in primary cultured granulosa cells. These results suggest that apoptosis occurs in granulosa cells but not cumulus cells in the atretic antral follicles in pigs, and ICE like protease (s) is involved in intracellular signal transmission of granulosa cell apoptosis of the atretic antral follicles in pigs.<br>

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