Changes of Cyclic AMP Levels and Phosphodiesterase Activities in the Rat Ovary

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  • WANG Zhengchao
    Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University
  • SHI Fangxiong
    Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University
  • JIANG Yong-qing
    Institute of Animal Sciences and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences
  • LU Li-zhi
    Institute of Animal Sciences and Veterinary Medicine, Zhejiang Academy of Agricultural Sciences
  • WANG Hao
    Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University
  • WATANABE Gen
    Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Tokyo University of Agriculture and Technology Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University
  • TAYA Kazuyoshi
    Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Tokyo University of Agriculture and Technology Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University

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Cyclic AMP (cAMP) is a second messenger that plays a critical role in follicular recruitment, development and luteinization in the mammalian ovary. The cellular level of cAMP is largely dependent on the activity of phosphodiesterase (PDE), which degrades cAMP into 5'-AMP. The present study was conducted to investigate the level of cAMP and the activity of cAMP-PDE in postnatal rats; immature rats during gonadotropin-primed follicular development, ovulation and luteinization; adult rats during normal estrous cycling; and aged rats that spontaneously developed persistent estrous (PE) by radioimmunoassay (RIA). All four rat models were confirmed by histological examination of one ovary and assayed using the other ovary by RIA. In the postnatal rats, the ovarian cAMP level was high on day 10 after birth, while ovarian cAMP-PDE activity was highest at 21 days of age. In the immature female rats, both the ovarian cAMP level and cAMP-PDE activity increased remarkably after treatment with equine chorionic gonadotropin (eCG), increased continuously 24 h after injection of human chorionic gonadotropin (hCG) for induction of ovulation and luteinization, and then declined significantly. In the adult rats during the normal estrous cycle, the ovarian cAMP levels were low on the day of estrus, and there were no significant changes in ovarian cAMP-PDE activity throughout the estrous cycle. In the PE rats, the ovarian cAMP levels were similar to those of the adult rats on the day of estrus but were lower than those on the other days of the estrous cycle; ovarian cAMP-PDE activity was lower than that in the adult rats on any day of the estrous cycle. Together, these findings indicate that the ovarian cAMP level and cAMP-PDE activity were regulated in a stage-dependent manner during ovarian follicular development, atresia and luteinization and providing evidences that cAMP and cAMP-specific PDEs are involved in these physiological processes.<br>

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