Birth of Normal Offspring from Mouse Eggs Activated by a Phospholipase C.ZETA. Protein Lacking Three EF-hand Domains

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  • NAKANISHI Tomoko
    Graduate School of Life and Environmental Sciences, University of Tsukuba Department of Molecular Biology, School of Life Science, Faculty of Medicine, Tottori University
  • ISHIBASHI Naoko
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • KUBOTA Haruka
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • INOUE Kimiko
    Institute of Physical and Chemical Research (RIKEN), Bioresource Center
  • OGONUKI Narumi
    Institute of Physical and Chemical Research (RIKEN), Bioresource Center
  • OGURA Atsuo
    Institute of Physical and Chemical Research (RIKEN), Bioresource Center
  • KASHIWABARA Shin-ichi
    Graduate School of Life and Environmental Sciences, University of Tsukuba
  • BABA Tadashi
    Graduate School of Life and Environmental Sciences, University of Tsukuba

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  • Birth of normal offspring from mouse eggs activated by a phospholipase Cζ protein lacking three EF-hand domains
  • Birth of normal offspring from mouse eggs activated by a phospholipase Cz protein lacking three EF hand domains

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Sperm-specific phospholipase C, PLCζ, is a candidate for the Ca2+ oscillation-inducing factor that is introduced into the ooplasm upon sperm-egg fusion. In addition to the 647-residue full-length PLCζ, s-PLCζ lacking the N-terminal 110 amino acids is known to be present in the mouse testis. In this study, we attempted to obtain full-term offspring from s-PLCζ-activated eggs by round spermatid injection. Metaphase II-arrested eggs injected with a high RNA concentration of s-PLCζ RNA normally developed to blastocysts. When the round spermatid nucleus was injected into telophase II-stage eggs previously activated by s-PLCζ RNA, three live offspring were successfully obtained by transfer of the developed 4-cell embryos to pseudopregnant mice. These three offspring all grew to be normal adults and reproduced healthy second-generation mice.<br>

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