精子発生・精子成熟過程でのマウスADAM3の生合成,プロセシング,および細胞内局在に関する解析

DOI 日本農学文献記事索引 Web Site Web Site PubMed 被引用文献10件 参考文献51件 オープンアクセス
  • KIM Ekyune
    Graduate School of Life and Environmental Sciences and Institute of Applied Biochemistry, University of Tsukuba
  • NISHIMURA Hitoshi
    Graduate School of Life and Environmental Sciences and Institute of Applied Biochemistry, University of Tsukuba Section of Molecular and Cellular Biology, University of California Davis
  • IWASE Shigeki
    Graduate School of Life and Environmental Sciences and Institute of Applied Biochemistry, University of Tsukuba
  • YAMAGATA Kazuo
    Graduate School of Life and Environmental Sciences and Institute of Applied Biochemistry, University of Tsukuba
  • KASHIWABARA Shin-ichi
    Graduate School of Life and Environmental Sciences and Institute of Applied Biochemistry, University of Tsukuba
  • BABA Tadashi
    Graduate School of Life and Environmental Sciences and Institute of Applied Biochemistry, University of Tsukuba

書誌事項

タイトル別名
  • Synthesis, Processing, and Subcellular Localization of Mouse ADAM3 during Spermatogenesis and Epididymal Sperm Transport
  • Synthesis, processing, and subcellular localization of mouse ADAM3 during spermatogenesis and epididymal sperm

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説明

To elucidate synthesis, processing, and subcellular localization of mouse ADAM3 (cyritestin) during spermatogenesis and epididymal sperm transport, we carried out immunoblotting and immunohistochemical analysis of testicular germ cells, and epididymal and vas deferens sperm, using affinity-purified anti-ADAM3 antibody. ADAM3 was initially synthesized as a 110-kDa precursor in round spermatids, and the precursor was then processed into a 42-kDa mature protein during the sperm transport into and/or once in the epididymis. The mature ADAM3 was localized on the anterior part of capacitated sperm heads and was rapidly removed from the head region during the calcium ionophore A23187-induced acrosome reaction. These results demonstrate that the mature form of ADAM3 is involved in the binding of sperm to the egg zona pellucida, not in the membrane fusion between sperm and egg.<br>

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