顕微授精後のブタ体外培養胚におけるWell of the Well (WOW)システムの影響

DOI 日本農学文献記事索引 Web Site Web Site PubMed 被引用文献8件 参考文献28件
  • TAKA Mikiko
    Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
  • IWAYAMA Hiroshi
    Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
  • FUKUI Yutaka
    Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine

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タイトル別名
  • Effect of the Well of the Well (WOW) System on In Vitro Culture for Porcine Embryos after Intracytoplasmic Sperm Injection

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For developmental competence of porcine embryos in vitro, it is important to improve the culture environment. The present study was performed to evaluate four different culture systems for in vitro matured porcine oocytes following intracytoplasmic sperm injection (ICSI); drop, well and two sizes of the well of the well (WOW) systems (500 and 1,000 μm in diameter). The cleavage rate on Day 2 and the mean cell number in blastocysts on Day 6 were not significantly different among the four treatments. However, the 1,000 μm WOW (24.6%) resulted in a significantly higher (P<0.05) blastocyst rate than those in the other culture systems (12.9, 14.8, and 7.1% for drop, well, and 500 μm WOW, respectively). The present study indicates that the microenvironment created by the 1,000 μm diameter WOW improves blastocyst production of in vitro matured porcine oocytes after ICSI, and that the effectiveness of the WOW system is dependent on the size (diameter) of the WOW.<br>

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