Attempt at Intracytoplasmic Sperm Injection of In Vitro Matured Oocytes in Common Minke Whales (Balaenoptera acutorostrata) Captured During the Kushiro Coast Survey

DOI Web Site Web Site 被引用文献2件 参考文献28件
  • FUKUI Yutaka
    Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
  • IWAYAMA Hiroshi
    Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
  • MATSUOKA Taiki
    Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
  • NAGAI Hiroki
    Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
  • KOMA Noriko
    Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine
  • MOGOE Toshihiro
    The Institute of Cetacean Research
  • ISHIKAWA Hajime
    The Institute of Cetacean Research
  • FUJISE Yoshihiro
    The Institute of Cetacean Research
  • HIRABAYASHI Masumi
    National Institute for Physiological Sciences
  • HOCHI Shinichi
    Faculty of Textile Science and Technology, Shinshu University
  • KATO Hidehiro
    Laboratory of Marine Biology, Tokyo University of Marine Science and Technology
  • OHSUMI Seiji
    The Institute of Cetacean Research

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The present study was conducted during the Kushiro Coast Survey in an attempt to produce common minke whale embryos. In Experiment 1, we attempted to determine the appropriate culture duration (30 or 40 h) for in vitro maturation (IVM) of immature oocytes using the Well of the Well method. In Experiment 2, and intracytoplasmic sperm injection (ICSI) was applied to matured oocytes from prepubertal and adult common minke whales after IVM culture (40 or 48 h), and then their embryonic development was assessed. In Experiment 1, the maturation rate of oocytes cultured for 40 h (30.4%) was significantly higher than that of oocytes cultured for 30 h (6.8%; P<0.01). In Experiment 2, a total of 35 and 46 immature oocytes derived from adult (n=2) and prepubertal (n=6) minke whales, respectively, were cultured for 40 or 48 h. The maturation rate in the oocytes from the adult whales (34.2%) tended to be higher than that of the oocytes from the prepubertal whales (19.6%), but there was no significant difference. Following ICSI, 3 out of the 10 inseminated and cultured oocytes from the adult whales cleaved (2-, 8-, and 16-cell stages); all of these oocytes had been matured for 40 in culture. However, these oocytes did not develop to further stages. Only one of the 6 oocytes derived from the prebuertal whales, IVM cultured for 40 h and inseminated, developed to the 4-cell stage. The present results indicate that a 40 h IVM culture produces significantly higher rates of in vitro maturation than a 30 h IVM culture for common minke whale oocytes. Following ICSI, some oocytes cleaved to the 16-cell stage, but no further development was observed.<br>

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