Lipopolysaccharide (LPS) Inhibits Steroid Production in Theca Cells of Bovine Follicles In Vitro : Distinct Effect of LPS on Theca Cell Function in Pre- and Post-selection Follicles
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- MAGATA Fumie
- Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, 0808555 Obihiro, Japan
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- HORIUCHI Maya
- Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, 0808555 Obihiro, Japan
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- MIYAMOTO Akio
- Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, 0808555 Obihiro, Japan
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- SHIMIZU Takashi
- Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, 0808555 Obihiro, Japan
書誌事項
- タイトル別名
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- Lipopolysaccharide (LPS) Inhibits Steroid Production in Theca Cells of Bovine Follicles <i>In Vitro</i>: Distinct Effect of LPS on Theca Cell Function in Pre- and Post-selection Follicles
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抄録
In postpartum dairy cows, lipopolysaccharide (LPS) derived from gram-negative bacteria such as Escherichia coli causes uterine inflammation and leads to ovarian dysfunction. The aim of this study was to determine the effect of LPS on steroid production in bovine theca cells at different stages of follicular development. Theca cells isolated from pre- and post-selection follicles (PRFs, <8.5 mm in diameter, and POFs, >8.5 mm in diameter, respectively) of bovine ovaries were exposed to LPS under luteinizing hormone (LH) conditions, estradiol (E2) conditions or both conditions in vitro. Bovine theca cells expressed the LPS receptor gene complex: Toll-like receptor 4 (TLR4), CD14 and MD2. LPS suppressed progesterone (P4) and androstenedione (A4) production with downregulation of steroidogenic enzyme transcripts when theca cells were stimulated with LH. By contrast, LPS did not affect P4 or A4 production when theca cells were stimulated with E2. P4 and A4 production in theca cells from PRFs was suppressed by LPS as early as at 48 h of culture, whereas the effect of LPS on theca cells from POFs was observed at 96 h of culture. The results demonstrate that LPS inhibits steroid production in theca cells under LH conditions. Moreover, theca cells from POFs showed a slower response to LPS compared with that of theca cells from PRFs, which might imply a distinct effect of LPS on follicles at different developmental stages. These findings suggest a possible mechanism of ovarian dysfunction and subsequent infertility in cows with endometritis.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 60 (4), 280-287, 2014
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206337687936
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- NII論文ID
- 130003390841
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- NII書誌ID
- AA10936678
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- COI
- 1:STN:280:DC%2BC2cnntVOltw%3D%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 025629482
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- PubMed
- 24769841
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可