Expression of Kruppel-Like Factor 6, KLF6, in Rat Pituitary Stem/Progenitor Cells and Its Regulation of the PRRX2 Gene
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- UEHARU Hiroki
- Laboratory of Molecular Biology and Gene Regulation, Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
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- HIGUCHI Masashi
- Institute of Reproduction and Endocrinology, Meiji University, Kanagawa 214-8571, Japan
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- NISHIMURA Naoto
- Laboratory of Molecular Biology and Gene Regulation, Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
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- YOSHIDA Saishu
- Laboratory of Molecular Biology and Gene Regulation, Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
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- SHIBUYA Shiori
- Laboratory of Molecular Biology and Gene Regulation, Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
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- SENSUI Kenta
- School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
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- KATO Takako
- Institute of Reproduction and Endocrinology, Meiji University, Kanagawa 214-8571, Japan
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- KATO Yukio
- Laboratory of Molecular Biology and Gene Regulation, Division of Life Science, Graduate School of Agriculture, Meiji University, Kanagawa 214-8571, Japan Institute of Reproduction and Endocrinology, Meiji University, Kanagawa 214-8571, Japan School of Agriculture, Meiji University, Kanagawa 214-8571, Japan
書誌事項
- タイトル別名
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- Expression of Krüppel-Like Factor 6, KLF6, in Rat Pituitary Stem/Progenitor Cells and Its Regulation of the PRRX2 Gene
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抄録
Paired-related transcription factors, PRRX1 and PRRX2, which are present in mesenchymal tissues and participate in mesenchymal cell differentiation, were recently found in the stem/progenitor cells of the pituitary gland of ectodermal origin. To clarify the role of PRRX1 and PRRX2 in the pituitary gland, the present study first aimed to identify transcription factors that regulate Prrx1 and Prrx2 expression. A promoter assay for the upstream regions of both genes was performed by co-transfection of the expression vector of several transcription factors, many of which are frequently found in the pituitary stem/progenitor cells. The results for the promoter activity of both genes showed expression in a cell type-dependent manner. Comprehensive comparison of transcriptional activity of several transcription factors was performed with CHO cells, which do not show Prrx1 and Prrx2 expression, and the results revealed the presence of common and distinct factors for both genes. Among them, KLF6 showed specific and remarkable stimulation of Prrx2 expression. In vitro experiments using an electrophoretic mobility shift assay and siRNA interference revealed a potential ability for regulation of Prrx2 expression by KLF6. Finally, immunohistochemistry confirmed the presence of KLF6 in the SOX2/PRRX2 double-positive stem/progenitor cells of the postnatal pituitary gland. Thus, the finding of KLF6 might provide a novel clue to clarify the maintenance of stem/progenitor cells of the postnatal pituitary gland.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 60 (4), 304-311, 2014
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206337724288
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- NII論文ID
- 130004053987
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- NII書誌ID
- AA10936678
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- COI
- 1:STN:280:DC%2BC2cjns1yqtA%3D%3D
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 025629524
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- PubMed
- 24881871
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可