Trichostatin A Promotes the Development of Bovine Somatic Cell Nuclear Transfer Embryos
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- LEE Min-Jung
- National Institute of Animal Science Department of Animal Sciences, Chungbuk National University
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- KIM Se-Woong
- National Institute of Animal Science
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- LEE Hyun-Gi
- National Institute of Animal Science
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- IM Gi-Sun
- National Institute of Animal Science
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- YANG Byoung-Chul
- National Institute of Animal Science
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- KIM Nam-Hyung
- Department of Animal Sciences, Chungbuk National University
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- KIM Dong-Hoon
- National Institute of Animal Science
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Description
We studied the effects of trichostatin A (TSA), a histone deacetylase inhibitor, on the development of bovine somatic cell nuclear transfer (SCNT) embryos by investigating (1) the optimal concentration and treatment time of TSA for development of bovine SCNT embryos, (2) the status of histone acetylation in TSA-treated and control SCNT embryos and (3) the expression of histone acetylation- and deacetylation-related genes in TSA-treated and control SCNT embryos. We observed that 50 nM TSA-treatment for 20 h following fusion resulted in more efficient in vitro development of bovine SCNT embryos to the blastocyst stage. In regard to histone H4K5 acetylation, half of the control SCNT embryos faintly displayed histone H4K5 signals 30 min after electrofusion, while most of the TSA-treated SCNT embryos displayed histone H4K5 signals within 30 min after electrofusion. Furthermore, the expressions of HDAC1 and HDAC2 in the blastocysts were significantly lower (P<0.05) in the TSA-treated SCNT than in the control SCNT. However, the expression of GCN5 and HAT1 did not differ between the TSA-treated and control SCNT. In conclusion, we demonstrated that TSA-treatment after SCNT in bovine embryos can dramatically improve the practical applications of current cloning techniques.<br>
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 57 (1), 34-42, 2011
The Society for Reproduction and Development
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Details 詳細情報について
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- CRID
- 1390001206337773184
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- NII Article ID
- 10027900965
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- NII Book ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL BIB ID
- 10991549
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed