Comparison of Commercial ELISA Blood Tests for Early Pregnancy Detection in Dairy Cows
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- PIECHOTTA Marion
- Clinic for Cattle, University of Veterinary Medicine Hannover
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- BOLLWEIN Julia
- Clinic for Cattle, University of Veterinary Medicine Hannover
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- FRIEDRICH Morten
- Department of Animal Science, University of Göttingen
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- HEILKENBRINKER Thomas
- Animal Health Department, Chamber of Agriculture Lower Saxony
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- PASSAVANT Chuck
- BioTracking, LLC
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- BRANEN Josh
- BioTracking, LLC
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- SASSER Garth
- BioTracking, LLC
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- HOEDEMAKER Martina
- Clinic for Cattle, University of Veterinary Medicine Hannover
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- BOLLWEIN Heinrich
- Clinic for Cattle, University of Veterinary Medicine Hannover
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Abstract
The objective of the present study was to compare two commercially available blood-based pregnancy tests, namely BioPRYN, an ELISA for pregnancy-specific protein B (PSPB), and an ELISA for pregnancy-associated glycoprotein (PAG), for early pregnancy diagnosis in dairy cattle using transrectal ultrasonography as a gold standard. Transrectal ultrasonography was conducted 26-58 days after artificial insemination (AI) in 197 cattle from 19 farms. Concurrently, a blood sample was collected for determination of serum PSPB and PAG. Transrectal palpation was performed approximately 120 days after AI to verify that pregnancy was maintained. For PSPB and PAG, there were no significant differences (P>0.05) in sensitivity (98.0 and 97.8%), specificity (97.1 and 91.2%), positive predictive values (99.3 and 97.8%), negative predictive values (91.9 and 91.2%) and accuracy (97.8 and 96.4%). In conclusion, the two blood pregnancy assays were equally efficacious and were highly accurate (based on transrectal ultrasonography as the gold standard).<br>
Journal
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 57 (1), 72-75, 2011
The Society for Reproduction and Development
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Details 詳細情報について
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- CRID
- 1390001206337782144
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- NII Article ID
- 10027901161
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- NII Book ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL BIB ID
- 10991632
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed