An immortalized steroidogenic goat granulosa cell line as a model system to study the effect of the endoplasmic reticulum (ER)-stress response on steroidogenesis
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- YANG Diqi
- Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, 712100, Shaanxi, China College of Veterinary Medicine, Northwest A&F University, 712100, Shaanxi, China
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- WANG Lei
- Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, 712100, Shaanxi, China College of Veterinary Medicine, Northwest A&F University, 712100, Shaanxi, China
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- LIN Pengfei
- Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, 712100, Shaanxi, China College of Veterinary Medicine, Northwest A&F University, 712100, Shaanxi, China
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- JIANG Tingting
- Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, 712100, Shaanxi, China College of Veterinary Medicine, Northwest A&F University, 712100, Shaanxi, China
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- WANG Nan
- Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, 712100, Shaanxi, China College of Veterinary Medicine, Northwest A&F University, 712100, Shaanxi, China
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- ZHAO Fan
- Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, 712100, Shaanxi, China College of Veterinary Medicine, Northwest A&F University, 712100, Shaanxi, China
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- CHEN Huatao
- Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, 712100, Shaanxi, China College of Veterinary Medicine, Northwest A&F University, 712100, Shaanxi, China
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- TANG Keqiong
- Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, 712100, Shaanxi, China College of Veterinary Medicine, Northwest A&F University, 712100, Shaanxi, China
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- ZHOU Dong
- Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, 712100, Shaanxi, China College of Veterinary Medicine, Northwest A&F University, 712100, Shaanxi, China
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- WANG Aihua
- College of Veterinary Medicine, Northwest A&F University, 712100, Shaanxi, China
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- JIN Yaping
- Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, 712100, Shaanxi, China College of Veterinary Medicine, Northwest A&F University, 712100, Shaanxi, China
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<p> With granulosa and theca cells, the ovaries are responsible for producing oocytes and secreting sex steroids such as estrogen and progesterone. Endoplasmic reticulum stress (ERS) plays an important role in follicle atresia and embryo implantation. In this study, goat granulosa cells were isolated from medium-sized (4–6 mm) healthy follicles. Primary granulosa cells were immortalized by transfection with human telomerase reverse transcriptase (hTERT) to establish a goat granulosa cell line (hTERT-GGCs). These hTERT-GGCs expressed hTERT and had relatively long telomeres at passage 50. Furthermore, hTERT-GGCs expressed the gonadotropin receptor genes CYP11A1, StAR, and CYP19A1, which are involved in steroidogenesis. Additionally, progesterone was detectable in hTERT-GGCs. Although the proliferation potential of hTERT-GGCs significantly improved, there was no evidence to suggest that the hTERT-GGCs are tumorigenic. In addition, thapsigargin (Tg) treatment led to a significant dose-dependent decrease in progesterone concentration and steroidogenic enzyme expression. In summary, we successfully generated a stable goat granulosa cell line. We found that Tg induced ERS in hTERT-GGCs, which reduced progesterone production and steroidogenic enzyme expression. Future studies may benefit from using this cell line as a model to explore the molecular mechanisms regulating steroidogenesis and apoptosis in goat granulosa cells.</p>
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 63 (1), 27-36, 2017
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206338189184
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- NII論文ID
- 130005332298
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 027985299
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- PubMed
- 27746409
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- PubMed
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