The Dynamin 2 inhibitor Dynasore affects the actin filament distribution during mouse early embryo development
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- WANG Qiao-Chu
- College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
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- LIU Jun
- College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
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- DUAN Xing
- College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
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- CUI Xiang-Shun
- Department of Animal Sciences, Chungbuk National University, Chungbuk 361-763, Korea
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- KIM Nam-Hyung
- Department of Animal Sciences, Chungbuk National University, Chungbuk 361-763, Korea
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- XIONG Bo
- College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
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- SUN Shao-Chen
- College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
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抄録
Dynamin 2 is a large GTPase notably involved in clathrin-mediated endocytosis, cell migration and cytokinesis in mitosis. Our previous study identified that Dynamin 2 regulated polar body extrusion in mammalian oocytes, but its roles in early embryo development, remain elusive. Here, we report the critical roles of Dynamin 2 in mouse early embryo development. Dynamin 2 accumulated at the periphery of the blastomere during embryonic development. When Dynamin 2 activity was inhibited by Dynasore, embryos failed to cleave to the 2-cell or 4-cell stage. Moreover, the actin filament distribution and relative amount were aberrant in the treatment group. Similar results were observed when embryos were cultured with Dynasore at the 8-cell stage; the embryos failed to undergo compaction and develop to the morula stage, indicating a role of Dynamin 2 in embryo cytokinesis. Therefore, our data indicate that Dynamin 2 might participate in the early embryonic development through an actin-based cytokinesis.
収録刊行物
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- Journal of Reproduction and Development
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Journal of Reproduction and Development 61 (1), 49-53, 2015
公益社団法人 日本繁殖生物学会
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詳細情報 詳細情報について
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- CRID
- 1390001206338276224
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- NII論文ID
- 130004703738
- 40020374289
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- NII書誌ID
- AA10936678
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- ISSN
- 13484400
- 09168818
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- NDL書誌ID
- 026186101
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- PubMed
- 25421092
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可