The Effects of Substance P on the Synovial Fibroblastic Cells from the Human Temporomandibular Joint

DOI Web Site Web Site 51 References

Bibliographic Information

Other Title
  • ヒト顎関節滑膜線維芽細胞におけるsubstance Pの影響
  • ヒト ガク カンセツカツマク センイ ガ サイボウ ニ オケル substance P ノ エイキョウ

Search this article

Abstract

Temporomandibular joint (TMJ) disorders (TMD) are often accompanied by pain and bone destruction ; however, the relationship between the pain and bone destruction is unclear. The nervous system has been implicated in the etiology and pathogenesis of joint diseases. Neuropeptides are secreted into the joint cavity, as a result of the stimulation of nerve endings. One of these neuropeptides, substance P (SP), is a member of the tachykinin family ; it is synthesized in the dorsal root ganglion (DRG) after sensory nerve fiber stimulation and transported in large part along axons to the terminal nerve endings. To clarify the effects of SP on bone destruction in TMD via synovial fibroblastic cells, we investigated the effects of SP on the proliferation and the RANKL expression of synovial fibroblastic cells from the human TMJ. Human synovial cells were isolated from TMJ synovial tissue using an outgrowth method and then cultured in α-minimal essential medium supplemented with antibiotics and containing 10% fetal bovine serum. The distribution of the neurokinin-1 receptor (NK_1-R ; SP receptor) in synovial fibroblastic cells was examined using immunofluorescence staining. The effect of SP on cell viability and proliferation was assessed using 5-bromo-2'-deoxyuridine (BrdU) uptake. The effects of SP on the receptor activator of nuclear factor kappa B ligand (RANKL) expression by synovial fibroblastic cells were examined using immunofluorescence staining. Immunohistochemistry demonstrated that synovial fibroblastic cells were positive for NK_1-R. The BrdU uptake of synovial fibroblastic cells was increased significantly after a 3-day incubation with 10^<-10>-10^<-6> M SP, compared with controls. Immunofluorescence staining demonstrated increased RANKL expression in synovial fibroblastic cells after adding SP, whereas no RANKL was expressed in synovial fibroblastic cells without adding SP. These results demonstrated that SP stimulates the proliferation of synovial fibroblastic cells and increases their expression of RANKL. These findings suggest that the secretion of SP in TMD causes both thickening of the synovium and increased osteoclast formation via the increased RANKL expression in the synovium.

Journal

References(51)*help

See more

Related Projects

See more

Keywords

Details 詳細情報について

Report a problem

Back to top