Development of the Cryo-TOF-SIMS/SEM System and Application for Topochemical Analysis of Plant Biomolecules
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- Aoki Dan
- Graduate School of Bioagricultural Sciences, Nagoya University
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- Kuroda Katsushi
- Forestry and Forest Products Research Institute
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- Hanaya Yuto
- Graduate School of Bioagricultural Sciences, Nagoya University
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- Saito Kaori
- Graduate School of Bioagricultural Sciences, Nagoya University Present address:Research Institute for Sustainable Humanosphere, Kyoto University
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- Takama Ruka
- Technical Center, Nagoya University
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- Matsushita Yasuyuki
- Graduate School of Bioagricultural Sciences, Nagoya University
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- Fukushima Kazuhiko
- Graduate School of Bioagricultural Sciences, Nagoya University
Bibliographic Information
- Other Title
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- Cryo-TOF-SIMS/SEMシステムの開発と植物生体分子のトポケミカル分析への適用
- Cryo-TOF-SIMS/SEM システム ノ カイハツ ト ショクブツ セイタイ ブンシ ノ トポケミカル ブンセキ エ ノ テキヨウ
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Abstract
Time-of-flight secondary ion mass spectrometry (TOF-SIMS) is a powerful tool to reveal the chemical distribution in many materials. We have applied TOF-SIMS to investigate the distribution of organic biomolecules and inorganic metals in plants. The positional, temporal, and quantitative information of water-soluble chemicals in a living state are essential information to elucidate many biological mechanisms. However, traditional quantification of water-soluble chemicals such as coniferin and sucrose depends on a bulk-analysis providing an approximate position of them. To analyze the position and quantity of the water-soluble chemicals in more detail, we developed the cryo-TOF-SIMS/SEM system. The system consists of a glove box, a cryo-SEM, and a cryo-TOF-SIMS, which were interconnected by a cryo-vacuum shuttle. By using the system, successive TOF-SIMS measurements and SEM observations are possible for the frozen-hydrated samples. We applied the system to freeze-fixed Ginkgo biloba and estimated the distribution of water-soluble chemicals: coniferin and sucrose. The quantity of coniferin and sucrose were evaluated by HPLC and ion chromatography, respectively. As a result of cryo-TOF-SIMS/SEM analysis, the secondary ion derived from coniferin and sucrose was unevenly distributed in phloem, tracheids, or ray cells.
Journal
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- Mokuzai Gakkaishi
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Mokuzai Gakkaishi 59 (6), 367-374, 2013
The Japan Wood Research Society
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Details 詳細情報について
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- CRID
- 1390001206351425408
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- NII Article ID
- 130004469053
- 40019886930
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- NII Book ID
- AN00240329
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- COI
- 1:CAS:528:DC%2BC2cXisFKluro%3D
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- ISSN
- 18807577
- 00214795
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- NDL BIB ID
- 025040273
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed