タイ国のサゴヤシ (Metroxylon spp.) のアイソザイム変異について

ブーンセルムスク スミット, 久島 繁, 石塚 晧造

doi:10.11248/jsta1957.39.229

Variation in sago palm in Thailand was studied based on zymograms of peroxidase, esterase, acid phosphatase and sorbitol dehydrogenase using polyacrylamide vertical slab gel electrophoresis (PAGE) and zymo-gram of esterase using disc gel isoelectric focusing (IEF) . Based on the zymograms of the five enzymes, isozyme variation among 6 individual sago palm plants from 15 populations each (90 in total) in southern Thailand were compared. Difference among zymograms of enzymes within the respective populations was limited. Difference among zymograms of enzymes in 90 sago palm plants was also limited. Major isozyme banding pattern of each enzyme among the plants was observed based on major bands. Zymograms of peroxidase and esterase by PAGE were classified into one type of isozyme banding patterns, namely 100% of type 1. Zymograms of acid phosphatase, sorbital dehydrogenase by PAGE showed two and three types, mainly type 1 (87%) and type 1 (95%), respectively. Zymograms of esterase by IEF were classified into one type, namely 100% of type 1. It was also observed that 80% of the 90 sago palm plants showed the same combination of isozyme banding patterns, namely type 1 for peroxidase zymogram by PAGE, type 1 for esterase zymogram by PAGE, type 1 for acid phosphatase by PAGE, type 1 for sorbitol dehydrogenase zymogram by PAGE and type 1 for esterase zymogram by IEF. The present results, showing a low isozyme variation among populations and growing regions, suggest that the genetic background of these sago palm plants is closely related to each other or the present sago individual plants may be derived from one ancestor or plants with very limited ancestry. Zymograms among 10 palm species including sago palm were also compared. It was suggested that sago palm plants could be distinguished from the other palm species by the present zymogram techniques and the 10 palm species could also be distinguished from each other.

タイ国のサゴヤシ (<I>Metroxylon</I> spp.) のアイソザイム変異について

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