Cellulase Exudaton by the Pine Wood Nematode-Detection of Activity in Its Crawling Track

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  • マツノザイセンチュウによるセルラーゼの分泌とその運動軌跡における検出
  • マツノザイセンチュウによるセルラーゼの分泌とその運動軌跡における検出〔英文〕
  • マツノザイセンチュウ ニ ヨル セルラーゼ ノ ブンピ ト ソノ ウンドウ キ

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Cellulase activity was detected in the supernatant of suspension of pine wood nematodes kept at 0°C for 12 hours, and the amount of the enzyme in the supernatant increased gradually in the following 36 hours. The isozyme pattern of the cellulase observed was similar to that detected in the homogenates of the nematodes. Nematodes placed on a polyacrylamide gel plate containing carboxymethyl cellulose left their crawling track on the gel plate by exuding cellulase outside. Feeding of the super-natant to pine seedlings induced partial necrosis of the needles. These results suggest that the pine wood nematodes exude cellulase, and induce the early symptom of the pine wilt disease.

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