Studies on the fractionation of clonorchiasis antigen responsible for the intradermal skin reaction

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  • 肝吸虫皮内反応抗原に関する研究
  • キモスイチュウ ヒ ナイ ハンノウ コウゲン ニ カンスル ケンキュウ

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Abstract

Adult worms of Clonorchis sinensis were treated with diethylether to remove lipids, disrupted with sonic vibrations and then centrifuged to obtain antigen KS. Fractionation of antigen KS on gel filtration on sephadex G 100 columns yielded an antigen KSC1 taht gave intense reactions in patients with clonorchiasis.<br>Diethylaminoethyl sephadex A 50 column chromatography of KSC1 produced a fraction KSCD4 which provoked strong positive skin tests in infected individuals but none in healthy individuals. No cross reactions were observed in individuals infected with other helminth than C. sinensis or in cases sufering from tuberculosis, hepatitis and diabetes etc.<br>Antigen KSCD4, containing 14γ of protein and 12.5γ of carbohydrate per ml, was added with 0.01M HCl and incubated at 70°C for 30 minutes to obtain antigen KSCDH which provoked less noticeable skin reactions. This fact suggests that the substance responsible for the skin reaction may be the protein component in the antigen KSCD4, though the further experiments are demanded.<br>It may be concluded that antigen KSCD4 can be used as test antigens for diagnosis of clonorchiasis in the field because they produce immediate intense reactions in individuals infected with C. sinensis and show no reaction in individuals without clonorchiasis but infected with helminth other than C. sinensis.

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