Fluorescence microscopic studies on the maturity of <i>Ascaris</i> larvae

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  • Terao Saburo
    Department of Hygiene, Jikei University School of Medicine

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  • 螢光顕微鏡法による回虫仔虫の成熟に関する研究
  • ケイコウ ケンビキョウホウ ニ ヨル カイチュウシチュウ ノ セイジュク ニ カンスル ケンキュウ

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By the fluorescence microscopy, the auther studied on the relation between the phenomenon of the appearance of autofluorescent granules in living larvae of Ascaris eggs and the maturity of the larvae.<br>Results obtained as follows:<br>In the 8-9 days larvae after the formation of embryos, fine granules (0.5-0.9μ in diameter), in which faint yellow autoflurescence was observed, were found chiefly in contact with the intestinal cells. Such larvae increased during 10-13 days after the embryo-formation, and the fluorescence color changed to golden yellow. These autofluorescent fine granules were scattered or standing in lines along the intestinal cells.<br>On the other hand, by the infection test with mice, it was confirmed that the infective larvae remarkably increased during 8-13 days after the embryo-formation. From the above observations, it was considered that the appearance of yellow autofluorescent fine granules showed the maturity of the larvae.<br>The infective power got to the peak during 16-30 days after the embryo-formation, and it fell down gradually after that, and the autofluorescence of the granules changed to golden yellow accompanied with transformation to round masses.

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