LYMPHOCYTE CYTOTOXICITY AGAINST HBsAg-COATED HEPATOCYTES IN PATIENTS WITH CHRONIC HEPATITIS

DOI PubMed
  • NONOMURA Akitaka
    Department of Pathology School of Medicine, Kanazawa University
  • NISHIMURA Isao
    Department of Internal Medicine, Kanazawa National Hospital
  • HARATAKE Joji
    Department of Pathology School of Medicine, Kanazawa University
  • KURUMAYA Hiroshi
    Department of Pathology School of Medicine, Kanazawa University
  • OOMORI Koji
    Department of Pathology School of Medicine, Kanazawa University
  • KATO Yasuhiro
    Department of Internal Medicine School of Medicine, Kanazawa University
  • KOBAYASHI Kenichi
    Department of Internal Medicine School of Medicine, Kanazawa University
  • SUGIOKA Goro
    Department of Internal Medicine, Kanazawa National Hospital
  • OHTA Goroku
    Department of Pathology School of Medicine, Kanazawa University

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Other Title
  • 慢性肝炎患者末しょう血リンパ球のＨＢｓ抗原被覆培養ヒト肝細胞に対する細胞障害性　Ｉ　　Ｔ‐ｃｅｌｌ　ｍｅｄｉａｔｅｄ　ｃｙｔｏｔｏｘｉｃｉｔｙの意義
  • I. T-cell mediated cytotoxicityの意義

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Abstract

T cells from about 30% of patients with chronic hepatitis showed significantly higher cytotoxicity against HBsAg-coated hepatocytes than healthy subjects, whereas non of the patients showed significant cytotoxicity against albumin-coated hepatocytes. Among patients with HBsAg-negative chronic hepatitis significant T-cell cytotoxicity against HBsAg-coated hepatocytes was observed in patients having cellular antibody to HBsAg detected by lymphocyte blast transformation responses. Similar positive T-cell mediated cytotoxicity was observed both in patients with convalescent acute B hepatitis and healed acute B hepatitis, suggesting that positive T-cell cytotoxicity in HBsAg-negative patients represents anamnestic responses to HBsAg of sensitized T lymphocytes and has no significant pathogenetic roles in hepatocyte destruction because HBsAg is seemed to be absent in their hepatocytes.<br>Significant T-cell cytotoxicity against HBsAg-coated hepatocytes was also found in patients with HBsAg-positive chronic hepatitis, having cellular antibody to HBsAg and low HBsAg titers in their sera. Levels of serum transaminase were significantly higher in patients showing positive T-cell cytotoxicity than those showing negative one.<br>In our experimental systems, HLA systems seemed not to be responsible for occurrence of the Tcell cytotoxicity reactions as seen in cytotoxic T-cell mediated cytotoxicity against virus-infected target cells. In consideration of the parallel occurrence of the positive T-cell cytotoxicity and cellular anti-HBs antibody, and the results of positive lymphotoxin assay in cytotoxicity-positive patients, it is suggested that T-cell mediated cytotoxicity in our systems seemed to be mediated by lymphokine producing T-cells as a result of delayed hypersensitivity reaction.<br>Non T-cells from both patients with chronic hepatitis and healthy subjects showed significantly higher cytotoxic activity to HBsAg-coated heaptocytes, when compared to those to albumin-coated hepatocytes. The cytotoxic values of non T-cells from patients with chronic hepatitis were higher than that of healthy subjects. This cytotoxic reactivity was interpreted as a spontaneous cellmediated cytotoxicity against HBsAg-coated hepatocytes.

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