LYMPHOCYTE CYTOTOXICITY AGAINST HBsAg-COATED HEPATOCYTES IN PATIENTS WITH CHRONIC HEPATITIS
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- NONOMURA Akitaka
- Department of Pathology School of medicine, Kanazawa university
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- NISHIMURA Isao
- Department of internal medicine, School of medicine, Kanazawa university
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- KURUMAYA Hiroshi
- Department of Pathology School of medicine, Kanazawa university
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- HARATAKE Joji
- Department of Pathology School of medicine, Kanazawa university
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- OOMORI Koji
- Department of Pathology School of medicine, Kanazawa university
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- KATO Yasuhiro
- Department of internal Medicine, National Kanazawa Hospital
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- KOBAYASHI Kenichi
- Department of internal Medicine, National Kanazawa Hospital
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- SUGIOKA Goro
- Department of internal medicine, School of medicine, Kanazawa university
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- OHTA Goroku
- Department of Pathology School of medicine, Kanazawa university
Bibliographic Information
- Other Title
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- 慢性肝炎患者末しょう血リンパ球のＨＢｓ抗原被覆培養人肝細胞に対する細胞障害性 ＩＩ ａｎｔｉ‐ＨＢｓ抗体処理による細胞障害性への影響
- II. anti-HBs抗体処理による細胞障害性への影響
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Abstract
Chang liver cells were coated with purified HBsAg using bisdiazotized benzidine reagents and cytotoxic activity of peripheral blood lymphocyes from patients with chronic hepatitis and healthy subjects against HBsAg-coated Chang cells was studied before and after treatment of HBsAg-coated targed cells with rabbit anti-HBs antibody.<br>Significant T-cell mediated cytotoxicity against HBsAg-coated Chang cells observed in patients with chronic hepatitis was significantly blocked after anti-HBs antibody treatment, indicating that cytotoxic reactions of T-cells were directed against HBsAg on the target cells.<br>Non T-cell cytotoxicity against HBsAg-coated hepatocytes in patients with chronic hepatitis was unchanged before and after treatment with anti-HBs antibody, whereas those of healthy subjects were significantly increased after treatment with anti-HBs antibody. Augumentation of non-T cell cytotoxicity after anti-HBs antibody treatment in healthy subjects was blocked by aggreagated human IgG, indicating that enhanced cytotoxic reactions were mediated by Fc-receptor positive lymhocytes. As a mechanism of the cytotoxicity was seemed to be an Antibody-dependent cell-mediated cytotoxicity. Failure of enhancement of non T-cell cytotoxicity after anti-HBs antibody treatment in patients with chronic hepatitis is considered to be the results of decreased K-cell activity in chronic hepatitis as we reported perviously.
Journal
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- Nippon Shokakibyo Gakkai Zasshi
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Nippon Shokakibyo Gakkai Zasshi 78 (10), 1947-1952, 1981
The Japanese Society of Gastroenterology