日本で分離された<i>Agrobacterium</i>属細菌株の抗生物質耐性

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  • Antibiotic Resistance of Agrobacterium Strains Isolated in Japan.
  • 日本で分離されたAgrobacterium属細菌株の抗生物質耐性〔英文〕
  • ニホン デ ブンリサレタ Agrobacteriumゾク サイキンカブ ノ コ

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To elucidate any of antibiotics usable for selecting bacterial cells harboring a binary vector to be employed for genetic manipulation of plants, we devised a spreading and dilution method on PDA plates for examining antibiotic resistance of Agrobacterium sp. The resistance of 46 strains of Agrobacterium mainly isolated in Japan were examined on 8 antibiotics, ampicillin, carbenicillin, chloramphenicol, kanamycin, rifampicin, streptomycin, tetracycline and vancomycin by the spreading and dilution method. No strains examined here were resistant to tetracycline. Almost all the strains were resistant to ampicillin and kanamycin and were sensitive or weakly resistant to rifampicin. While the strains belonging to biovar 1 except for two strains exhibited high resistance to vancomycin, the strains belonging to biovar 2 except for groups 3 and 4 exhibited high resistance to carbenicillin but sensitivity or weak resistance to vancomycin. On the other hand, each of the strains exhibited various level of resistance to chloramphenicol and streptomycin even if they belonged to the same biovar. We therefore conclude that tetracycline is the best antibiotic for selecting cells of Agrobacterium, examined in the present study, harboring binary vectors. Moreover, we suggest that the results of antibiotic resistance in each biovar are usable for a supplementary classification of Agrobacterium.

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