An observation method of the growth stage of Botrytis cinerea on primula petals.

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  • プリムラ花弁上での灰色かび病菌の生育過程の観察法
  • プリムラ カベンジョウ デ ノ ハイイロカビビョウキン ノ セイイク カテイ

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Abstract

For observing the growth stages of Botrytis cinerea, a simple method using petals of primula or petunia was developed. When the petals were inoculated with conidial suspension prepared from the culture of B. cinerea, conidial germination began by 5 hr after inoculation. Hyphal penetration occurred by 8 hr, when conidial germination was attained to about 50%. Conidiophores were developed within 3 to 4 days after inoculation, and sporulation was observed on the following day. The symptoms appeared by 11 hr after inoculation, and the number of lesions reached the maximum level by 16 hr (about 180 lesions at inoculum concentration of 105 conidia/ml). Since the life cycle of the fungus is so quick and symptom appears so rapidly on petals, the present method is expected to be applicable for screening chemicals.

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