ネパールのカンキツグリーニング病病原体リボソームDNAの検出と解析により明らかになったマンダリンにおけるグリーニング病の典型的病徴
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- 大津 善弘
- 果樹試験場保護部
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- 中島 一雄
- 国際農林水産業研究センター生物資源部
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- PROMMINTARA Maitree
- Plant Pathology and Microbiology Division, Department of Agriculture
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- 富安 裕一
- 国際協力事業団ネパール園芸開発プロジェクト
書誌事項
- タイトル別名
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- Typical Symptoms of Citrus Greening on Mandarin Trees in Nepal, Supported by Detection and Characterization of Ribosomal DNA of the Causal Organisms.
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Citrus greening disease caused by greening organisms (GOs; Liberobacter spp.) is one of the most destructive diseases of citrus in Nepal and Thailand. The 16S rDNA fragments of the GOs were detected by PCR in leaves showing one of the seven typical symptoms: mottling (type I), chlorosis with green netlike veins (type II), severe chlorosis with green main veins (type III), pale green on young leaves (type IV), vein yellowing (type V), vein corking (type VI) and yellow blotching (type VII). Leaves were collected from GO-infected ‘Suntala’ mandarin trees in Nepal. Sequence analysis of the 16S rDNA fragments and the 16S/23S intergenic spacer regions of Nepalese and Thai isolates of GOs indicated that these isolates were closely related to Indian and Chinese isolates of L. asiaticum. Symptom types III, V and VI were considered to be optimum for PCR diagnosis because of the high amount of GO DNA amplified. The accuracy of diagnosis by the naked eye can be improved by observing as many leaves as possible with all seven typical symptoms of greening on the same mandarin tree. Symptom types III, V and VII may be common to mandarin and sweet orange, because these symptoms are very similar to those reported on sweet orange in South Africa.
収録刊行物
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- 日本植物病理学会報
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日本植物病理学会報 64 (6), 539-545, 1998
日本植物病理学会
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キーワード
詳細情報 詳細情報について
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- CRID
- 1390001206401801472
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- NII論文ID
- 110002732871
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- NII書誌ID
- AN0019269X
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- COI
- 1:CAS:528:DyaK1MXhvVOjsbo%3D
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- ISSN
- 18820484
- 00319473
- http://id.crossref.org/issn/00319473
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- NDL書誌ID
- 4663779
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可