Simplified differentiation of three causal agents of potato common scab by TLC using secondary metabolites.

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  • MANABE K.
    Faculty of Agriculture, Tokyo University of Agriculture
  • TASHIRO N.
    Saga Prefectural Fruit Tree Experiment Station
  • NEGISHI H.
    Faculty of Agriculture, Tokyo University of Agriculture
  • SUYAMA K.
    Faculty of Agriculture, Tokyo University of Agriculture
  • MATSUYAMA N.
    Faculty of Agriculture, Tokyo University of Agriculture

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Other Title
  • 二次代謝産物を指標にした３種のジャガイモそうか病菌のＴＬＣ法による簡易識別
  • 2ジ タイシャ サンブツ オ シヒョウ ニ シタ 3シュ ノ ジャガイモソウ カ ビョウキン ノ TLCホウ ニ ヨル カンイ シキベツ

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New benchmarks were developed for differentiating Streptomyces scabies, S. acidiscabies and S. turgidiscabies, that cause potato common scab. Secondary metabolites of the pathogens were extracted directly with methanol-diethylether (2:1, v/v) or acetonitrile from a solid Bennett's medium after 5 days of culture at 25°C. The extracts were spotted onto a precoated silica gel TLC plate (Merck Co., Si60, 0.25mm), which was then developed at 25°C for 90min, with chloroform-methanol-acetone-acetic acid-distilled water (55:20:4:4:2, v/v). The characteristic spots were detected by UV irradiation at 365nm, and the resultant chromatograms were recorded with a digital camera and analyzed with a personal computer. Because chromatograms are specific, three causal agents of potato common scab disease can be rapidly differentiated.

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