Immunological Detection of endoPolygalacturonase Secretion by Fusarium oxysporum in Plant Tissue and Sequencing of Its Encoding Gene.
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- ARIE Tsutomu
- Institute of Physical and Chemical Research (RIKEN)
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- GOUTHU Satyanarayana
- Institute of Physical and Chemical Research (RIKEN)
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- SHIMAZAKI Satoshi
- Kyowa Seed Co.
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- KAMAKURA Takashi
- Institute of Physical and Chemical Research (RIKEN)
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- KIMURA Makoto
- Institute of Physical and Chemical Research (RIKEN)
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- INOUE Mitsuko
- Faculty of Agriculture, Meiji University
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- TAKIO Koji
- Institute of Physical and Chemical Research (RIKEN)
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- OZAKI Akira
- Institute of Physical and Chemical Research (RIKEN)
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- YONEYAMA Katsuyoshi
- Faculty of Agriculture, Meiji University
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- YAMAGUCHI Isamu
- Institute of Physical and Chemical Research (RIKEN)
Bibliographic Information
- Other Title
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- <i>Fusarium oxysporum</i>の植物組織中におけるエンドポリガラクツロナーゼの分泌の免疫学的観察と本酵素をコードする遺伝子の単離
- Immunological Detection of endo-Polygal
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Abstract
Fusarium oxysporum, a soilborne phytopathogenic fungus, produces endo polygalacturonase (PG) which has been suggested to be related to disease development. A polyclonal antibody (PAb) APG1 was prepared against purified PG from F. oxysporum f. sp. lycopersici race 2. By direct tissue-blotted immunobinding assay (DT-IBA) with the antibody, PG production by the pathogen in the stem of the host plant was confirmed. Degenerate primers were designed based on partial amino acid residue information for the PG protein from the fungus: part of the PG-encoding gene was obtained by PCR. Using TAIL-PCR, the complete gene encoding PG was cloned and sequenced. The structural gene comprises 1318 bp coding for 371 amino acids with a putative signal peptide of 22 amino acids, and the open reading frame is interrupted by four introns of 47, 51, 50, and 54bp. The deduced amino acid sequence of the mature protein showed 82.9, 29.0, 27.6, 14.2, and 26.9% homology with those of F. moniliforme PG, Cochliobolus carbonum PGNI, Aspergillus niger PG, A. oryzae PG, and Sclerotinia sclerotiorum PGI, respectively.
Journal
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- Japanese Journal of Phytopathology
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Japanese Journal of Phytopathology 64 (1), 7-15, 1998
The Phytopathological Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390001206402102400
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- NII Article ID
- 110002732348
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- NII Book ID
- AN0019269X
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- COI
- 1:CAS:528:DyaK1cXjvFWgsL0%3D
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- ISSN
- 18820484
- 00319473
- http://id.crossref.org/issn/00319473
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- NDL BIB ID
- 4425153
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed