インドール酢酸とイネ・カルス組織の培養〓液との組合わせによるイネ白葉枯病菌の生育阻害

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  • Growth inhibition of Xanthomonas oryzae by combination of IAA and culture filtrate of rice callus tissues.
  • インドール酢酸とイネ・カルス組織の培養濾液との組合わせによるイネ白葉枯病菌の生育阻害〔英文〕
  • インドール サクサン ト イネ カルス ソシキ ノ バイヨウ ロエキ ト ノ

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Rice callus tissues were induced from four rice cultivar's sprouts (Kinmaze, Kogyoku, Te-tep, and Waseaikoku No.3). It was observed that these calli secreted high molecular weight antibiotic components against Xanthomomas oryzae into the medium during their growth, when the bacterial growth was tested on the agar medium supplemented with indole-3-acetic acid (IAA). The influences of addition of plant hormones to Murashige-Skoog's medium on growth of the rice calli and on secretion of the antibiotic component from the callus tissues were examined after incubation for 3 weeks at 27C. Callus tissues of Kinmaze and Kogyoku secreted relatively small amounts of the antibiotic component when they grew on the medium with added low concentration (0.05ppm or less) of plant hormones (IAA, 2, 4-D, NAA or kinetin). However, the antibiotic activity in the culture media of Kinmaze and Kogyoku callus tissues increased almost as much as that found in the media of Te-tep and Waseaikoku No.3 callus tissues, when they grew on the media supplemeted with higher concentration (0.5 to 20ppm) of 2, 4-D. On the other hand, the degree of secretion of the active component from Te-tep or Waseaikoku No.3 callus tissues was relatively high even when they grew on the medium contained low concentration (0.05ppm or less) of plant hormones, and was mostly 10 to 100 times as much as that from Kinmaze or Kogyoku when they grew on the medium with added higher concentration of IAA, NAA or kinetin. The antibiotic component seemed to be one of IAA oxidizing enzymes, because it was a high molecular weight substance of around 32, 000 daltons, it required Mn++ as a cofactor, and the activity was stimulated by addition of monohydric phenol.

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