培地上におけるクワ又枯病菌の分生胞子形成

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  • Sporulation of <i>Stigmina mori</i> on Culture Media
  • バイチジョウ ニ オケル クワマタ コビョウキン ノ ブンセイ ホウシ ケイセ

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Stigmina mori, the causal fungus of mulberry branch blight, does not produce conidia on standard laboratory media. Monoconidial isolates of the fungus were cultured at alternating temperature of 20 to 5C under alternating 12-hr light-dark cycles (light 6:00-18:00, 20C; dark 18:00-6:00, 5C). Profuse sporulation occurred during growth for 50 to 60 days on epidermis removed mulberry shoot bark decoction agar plus 0.2% glucose. However, mulberry shoot bark decoction agar with epidermis yielded few conidia. Best results were also obtained when the fungus was cultured on mulberry shoot bark agar medium at alternating temperature of 15 to 5C under alternating 12-hr light-dark cycles (light 6:00-18:00, 15C; dark 18:00-6:00, 5C). Abundant sporulation occurred during growth for 27 to 32 days, Apparently there is a nutritional factor in mulberry shoot cortex tissues that promotes sporulation of S. mori. Conidia were produced at ends of hyphae, on short lateral hyphal branches, and also directly on hyphae. “Lesion-like sporulating area” was formed on mulberry shoot agar and mulberry shoot bark agar medium. There were significant differences in sporulation capacity between monoconidial isolates. Isolates S201-4, S201-5 and SE79-2 produced much more conidia than other isolates tested.

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