Detection of mulberry dwarf and onion yellows phytoplasmas by PCR from vector insects and nonvector insects.

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  • NAKAJIMA S.
    Koibuchi College of Agriculture
  • NISHIMURA N.
    Koibuchi College of Agriculture
  • MATSUDA I.
    National Agriculture Research Center National Institute for Agro-Environmental Sciences
  • SHIOMI T.
    National Agriculture Research Center National Institute for Agro-Environmental Sciences
  • NAMBA S.
    Graduate School of Frontier Sciences, University of Tokyo
  • TSUCHIZAKI T.
    Koibuchi College of Agriculture

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Other Title
  • クワ萎縮病及びタマネギ萎黄病ファイトプラズマのＰＣＲ法による媒介昆虫及び非媒介昆虫からの検出
  • クワ イシュクビョウ オヨビ タマネギイオウビョウ ファイトプラズマ ノ PCRホウ ニ ヨル バイカイ コンチュウ オヨビ ヒバイカイ コンチュウ カラ ノ ケンシュツ

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Abstract

Phytoplasmas were detected by PCR in vector leafhoppers Hishimonus sellatus and Macrosteles striifrons of onion yellows phytoplasma (OYP), and in a vector leafhopper H. sellatus of mulberry dwarf phytoplasma (MDP), respectively, after they fed on infected plants or were injected with the extract from infective insects. MDP was not detected by PCR in nonvector M. striifrons fed on infected plants, but was detected in preparations from whole bodies, headless bodies and heads of M. striifrons injected with the extract from infective insects. M. striifrons injected with infective MDP failed to transmit MDP to test plants.

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