Physicochemical and serological properties of viruses from bamboo culm rust, Stereostratum corticioides.

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  • メダケ赤衣病菌ウイルスの理化学的ならびに血清学的性状
  • メダケ赤衣病菌ウイルスの理化学的ならびに血清学的性状〔英文〕
  • メダケ アカゴロモビョウキン ウイルス ノ リカガクテキ ナラビニ ケッセイガ

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Abstract

Properties of viruses from bamboo culm rust fungus, Stereostratum corticioides (Berk. et Br.) Magnus were investigated. S. corticioides virus (StcV) was purified by chloroform clarification, polyethylene glycol precipitation and differential centrifugations. The sucrose density gradient centrifugation yielded a single band formed by spherical viruses about 40nm in diameter. The buoyant densities of intact and empty virus particles in CsCl were about 1.39 and 1.29g/cm3, respectively. Two major capsid proteins with molecular weights of about 92, 000 and 80, 000 daltons were characterized by SDS-polyacrylamide gel electrophoresis. The viral nucleic acid was shown to be double-stranded (ds) RNA by enzymatic tests and molecular weight of the dsRNA was estimated about 3.5×106 daltons by 1% agarose gel electrophoresis. Antiserum against StcV was prepared by immunizing a rabbit. Immune electron microscopy (IEM) showed that this antiserum reacted with viruses from different origins of S. corticioides, suggesting that serologically similar viruses are widespread. On the other hand, morphologically similar viruses from Puccinia allii, P. hordei, P. miscanthi, P. recondita and Coleosporium asterum did not react with StcV antiserum. It was shown that morphologically similar but serologically distinct viruses existed among rust fungi.

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