異なった昆虫媒介性をもつ2種のカリフラワーモザイクウイルス間の<i>in vitro</i>組換え体の特性

DOI 日本農学文献記事索引 Web Site 被引用文献1件

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タイトル別名
  • Characterization of In Vitro Recombinant Cauliflower Mosaic Viruses in Aphid Transmissibility.
  • 異なった昆虫媒介性をもつ2種のカリフラワーモザイクウイルス間のin vitro組換え体の特性〔英文〕
  • コトナッタ コンチュウ バイカイセイ オ モツ 2シュ ノ カリフラワー モザ

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抄録

Aphid transmissible Japanese isolate of caulifiower mosaic virus M (CaMV-M) was cloned into pUC19 and its ORF II region was sequenced. Comparison of this sequence with that of CM1841 (non-aphid transmissible isolate of CaMV) showed that there were nine base changes, four of which gave rise to alterations in the predicted amino acid sequence. Characterization of in vitro recombined hybrid viruses between CaMV-M and CM1841 showed that 5' half of ORF II was responsible for the lack of aphid transmissibility in CM1841 and this region influenced the amount of accumulation of ORF II product in the infected leaves.

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