トマト黄化えそウイルス粒子中に存在する相補的RNA
書誌事項
- タイトル別名
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- The Presence of Three Pairs of Possibly Complementary RNA Species in Isolated Nucleocapsid Material of Tomato Spotted Wilt Virus.
- トマト黄化えそウイルス粒子中に存在する相補的RNA〔英文〕
- トマト オウカエソ ウイルス リュウシチュウ ニ ソンザイスル ソウホテキ R
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P isolate of tomato spotted wilt virus (TSWV-P) was purified by ultracentrifugation followed by metrizamide isopycnic density-gradient. Nucleocapsid purified after Triton X-100 treatment was disrupted with 1% SDS and analyzed by 1% agarose gel electrophoresis. RNA patterns were indistinguishable from those obtained from intact particles and both contained six single-stranded RNA species (ss1-ss6). When RNAs were extracted by phenol and precipitated by ethanol, three double-stranded RNA species (ds1-ds3) were appeared. The ds1, ds2 and ds3 was formed after annealing ss1 with ss2, ss3 with ss4 and ss5 with ss6, respectively. Glyoxal treatment rendered ds1-ds3 to three single-stranded RNA segments (L, M and S RNA). In Northern blot hybridization analyses using three glyoxalated single-stranded RNA segments as probes, S RNA hybridized with ss5 and ss6, in addition to ds3. M RNA hybridized with ss3, ss4 and ds2. L RNA also hybridized with ss1, ss2 and ds1. Moreover, S RNA segment, ss5, ss6 and ds3, hybridized with oligonu-cleotide probes of both polarities representing 3′ end sequence of S RNA segment and an internal part on N protein coding region. A probe of viral strand on N protein coding region, however, hybridized weakly to S RNA segment and seemed not to react to ss5. Interestingly, the amount of viral strand was about 4 times larger than the amount of viral complementary strand in S RNA species, as judged from reactivity of hybridization using strand-specific probes. These results indicate that three different-sized RNA segments are present in both viral and viral complementary strands in the nucleocapsid of TSWV.
収録刊行物
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- 日本植物病理学会報
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日本植物病理学会報 58 (3), 393-404, 1992
日本植物病理学会
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詳細情報 詳細情報について
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- CRID
- 1390001206404140800
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- NII論文ID
- 110002735637
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- NII書誌ID
- AN0019269X
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- ISSN
- 18820484
- 00319473
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- NDL書誌ID
- 3799874
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
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- 使用不可