コンジュゲーションもしくはエレクトロポレーションによるＡｇｒｏｂａｃｔｅｒｉｕｍ　ｒｈｉｚｏｇｅｎｅｓＭＡＦＦ０３‐０１７２４株が保有するｐＲｉ１７２４の移行とその解析

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  • Transfer and Analysis of pRil724 in Agrobacterium rhizogenes Strain MAFF03-01724 by Conjugation or Electroporation.
  • コンジュゲーション モシクワ エレクトロポレーション ニ ヨル Agrobact

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A root-inducing plasmid, pRi1724, in Agrobacterium rhizogenes strain MAFF03-01724 (mikimopine type) isolated in Japan, was combined with a kanamycin resistant gene. The resulting plasmid called pRi1724:: kan was transferred from strain 1724 to other Agrobacterium strains by bacterial transconjugation or electroporation. A. tumefaciens strain C58C1 harboring pRi1724:: kan had abilities such as induction of hairy roots, biosynthesis of mikimopine in infected cells and degradation of mikimopine. We could deduce that the size of pRi 1724 ranged from 205 to 210 kb from results of electrophoresis of fragments from pRi1724:: kan digested by restriction enzymes.

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