Heterothallism in <i>Fomitiporia</i> sp. causing Japanese pear dwarf.

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  • KANEKO Y.
    Chiba Prefectural Agriculture and Forestry Research Center
  • NAKAMURA H.
    NARO Institute of Fruit Tree Science
  • USHIO S.
    Chiba Prefectural Agriculture and Forestry Research Center

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Other Title
  • ナシ萎縮病菌<i>Fomitiporia</i> sp.におけるヘテロタリズム
  • ナシ萎縮病菌Fomitiporia sp.におけるヘテロタリズム
  • ナシ イシュク ビョウキン Fomitiporia sp.ニ オケル ヘテロタリズム
  • Heterothallism in Fomitiporia sp. causing Japanese pear dwarf.

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Abstract

We determined the mating system of the pathogen of Japanese pear dwarf, Fomitiporia sp., in pairing tests of single basidiospore isolates from two vegetative strains isolated from decayed wood and from another vegetative strain from a fruiting body produced on a Japanese pear tree. Each basidiospore usually had a single nucleus. Number of nuclei per hyphal cell varied from two to six for vegetative strains and one to six for single basidiospore isolates. Thus, it was difficult to distinguish between primary and secondary hyphae based on nucleus number. In pairing tests using 15–20 single basidiospore isolates from each strain, compatible reactions were characterized by tuft formation at the junction between two mycelia. Tuft isolates, analyzed by inter-simple sequence repeat (ISSR) markers using a PCR primer (GTG)5, were found to be composed of secondary hyphae. In incompatible pairing reactions, no tufts formed. Mating patterns based on tuft reactions were complex and ambiguous for determining polarity; however, the fungus was inferred to be bipolar or tetrapolar. Most inter-strain combinations were compatible. In conclusion, the pathogen of Japanese pear dwarf, Fomitiporia sp., was predominantly heterothallic.<br>

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