Manufacturing of Glucose from Starch by Amylases of <I>Corticium rolfsii</I>

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  • TACHIBANA SADAO
    Tokushima Agricultural High School
  • SATO MASAYUKI
    Department of Agricultural Chemistry, Faculty of Agriculture, Kagawa University
  • KAJI AKIRA
    Department of Agricultural Chemistry, Faculty of Agriculture, Kagawa University

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  • ?EＣｏｒｔｉｃｉｕｍ　ｒｏｌｆｓｉｉのアミラーゼ剤によるでんぷんからグルコースの製造
  • Corticium rolfsii ノ アミラーゼザイ ニヨル デンプン カラ

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Abstract

The crude glucoamylase preparation was obtained from the culture filtrate of Corticium rolfsiiIFO 4878 when starch was used as carbon source. The preparation contained typical glucoamylase with an optimum for hydrolytic activity at pH 3.0 toward corn starch. The enzyme was unusually acid stable; even after storage at pH 2.0 or 3.0 and 30°C for 24hr, 85% of enzymic activity remained. The ratio of hydrolysis was about 100% when the crude enzymes acted on 40% corn starch together with bacterial α-amylase. The glucoamylase preparation of C. rolfsii might be available for manufacturing of glucose from starch as well as commercial glucoamylase preparations.

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