固定化酵素の製造　ＶＩＩＩ　　粒状固定化インベルターゼ

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  • Studies on the Preparation of Immobilized Enzymes by Radiopolymerization
  • コテイカ コウソ ノ セイゾウ ニカンスルケンキュウ 8 リュウジョウ コテイ
  • (第8報)粒状固定化インベルターゼについて

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Invertase was immobilized in an bead shaped polymer(0.5-1mm diameter)by radiocopolyme-rization of some synthetic monomers at the frozen temperature. Some of the basic characteristics were investigated and enzyme column was tested.<BR>1) Immobilized invertase preparations having high retained activities(60-90%)were prepared by polymerizing some monomers without acrylamide.<BR>2) The optimum reaction temperature was about 65°C being 5°C higher than that of the native invertase.<BR>3) Heat stability was greatly improved. After heating at 70°C for 12min, immobilized invertase hold sti11 70% of the original activity but native one had 40%.<BR>4) Immobilized invertase was more resistant to aniline, urea and Hg++ but on the contrary, more sensitive to SDS, guanine and Cu++.<BR>5) The Km value was 2.45×10-2 mole and 1.75×10-2 mole for immobilized and native enzyme respectively.<BR>6) The activation energy was increased by 0.9Kcal/mole after immobilization.<BR>7) When used as immobilized enzyme column, it was found to be more advantageous to increase the concentration of substrate and enzyme in the entrapping polymer.<BR>8) 30% sucrose solution was continously charged in to the column at the flow rate of SV 1. The substrate was completely decomposed up to 28 or 50 days.<BR>9) There were no big differences on the basic characteristics between bead shaped and membranous immobilized invertase preparations except for the behavious to some inhibitors.

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