茶のヌクレオチド　ＩＩＩ　　茶葉の核酸分解酵素系によるＲＮＡの分解

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  • Studies on the Nucleotides in Tea
  • チャ ノ ヌクレオチド ニカンスルケンキュウ 3 チャハ ノ カクサン ブンカ
  • (第3報)茶葉の核酸分解酵素系によるRNAの分解

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Two types of endonucleases were separated from the crude enzyme preparation of tea leaves by DEAE-cellulose column chromatography. One of them released mainly purine 5'-nucleotides and the other produced 2', 3'-cyclic nucleotides, when each enzyme fraction was incubated with yeast RNA.<BR>The latter enzyme, ribonuclease(RNase)was further purified by Sephadex G-100 gel filtration. Yeast RNA was rapidly degraded to a mixture of 2', 3'-cyclic nucleotides and oligonucleotides by this enzyme, and the cyclic nucleotides were slowly hydrolyzed to the corresponding 3'-nucleotides.<BR>From the properties of tea leaf RNase, it was considered that 2'- and 3'-nucleotides in black tea were produced by the both actions of the enzymes such as RNase and PDase and possibly non-enzymic hydrolysis of the cyclic nucleotides, which were liberated from the tea RNA by the action of RNase.

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