書誌事項
- タイトル別名
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- Induction of a high vitamin B12 productive Natto strain through protoplast fusion.
説明
To breed a high vitamin Bl2 producible natto strain, protoplast fusion was done betweenBacillus natto, Takahashi No.2 (Arg-mutant), and a vitamin B12 productive strain, Bacillus megaterium IAM 1166 (Try-mutant). Lysozyme of 250μg/ml and 500μg/ml wasadopted for the protoplast preparation of B. natto Takahashi No.2 and B. megaterium IAM1166, respectively, with 10% sucrose at 42°C for 30min. Protoplast fusion was carried outwith 40% polyethyleneglycol 6000, and the regeneration was performed on SucroseGlutamateminimal medium containing 10% sucrose and 3% polyvinylpyrolidon. Preservation of thestringiness and vitamin B12 productivity of the protoplast strains were deeply affected by theconcentrations of lysozyme and sucrose. Three fusants which were capable of producingstringiness and vitamin B12 were isolated on SG medium, and the strong stringiness on asteamed soybean by them were also observed in a similar manner as B. natto.
収録刊行物
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- 日本食品工業学会誌
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日本食品工業学会誌 35 (3), 154-159, 1988
社団法人 日本食品科学工学会
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詳細情報 詳細情報について
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- CRID
- 1390001206406162432
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- NII論文ID
- 130003788721
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- ISSN
- 00290394
- http://id.crossref.org/issn/00290394
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
- OpenAIRE
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- 抄録ライセンスフラグ
- 使用不可
