リアクター型バイオセンサシステムによるマイクロ波乾操茶葉中のビタミンＣ計測

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  • Determination of L-Ascorbic Acid in Green Tea with an Enzyme Reactor Electrode System.
  • リアクターガタ バイオセンサ システム ニヨル マイクロハ カンソウ チャハチ

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A continuous system for the determination of ascorbic acid with an enzyme reactor electrode was developed and applied to the measurement of ascorbic acid in green tea produced by a microwave heating (2450MHz) and a conventional method. The system consisted of an enzyme reactor, an oxygen electrode, an A/D converter, a microcomputer and a peristaltic pump. The reactor (φ2×50mm) was packed with ascorbate oxidase immobilized on chitosan porous beads. One assay could be completed within 3min. The limit of detection was 1mg/100ml, the relative error was within 2%(n=40) and the response was linear up to 1000mg/100ml. The immobilized enzyme was sufficiently stable for at least 3 months at room temperature. The results for tea extracts correlated satisfactorily well with those obtained by the enzyme method (F-kit) and 2, 6-dichlorophenolindophenol method. These results suggest that the system proposed is a simple, rapid and economical method for the determination of ascorbic acid in foods. Furthermore. it was proved a larger amount of ascorbic acid remained in the green tea produced by microwave heating compared with that produced by a conventional method.

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