A Simple Method for Preparation of Cellooligosaccharides
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- SASAKI TAKASHI
- National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
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- TANAKA TAKASHI
- National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
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- KAINUMA KEIJI
- National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
Bibliographic Information
- Other Title
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- セロオリゴ糖の簡易な調製法
- セロ オリゴトウ ノ カンイ ナ チョウセイホウ
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Description
Cotton cellulose powder (5g) was dissolved in 20ml solution of conc. H2SO4-conc. HCl (1:1) and then 60ml of conc. HCl was added, and it was hydrolyzed partially at 20°C for 5hr. The produced cellooligosaccharides were recovered by cold acetone fractionation from 70 to 95%, and the yield was about 250mg in tatal. The cellooligosaccharides were separated to G1, G2, G3, G4 and G5 by paper chromatography with n-butanol-pyridine-water (4:4:1) and the elution profile of the cellooligosaccharides by Bio-Gel P-4→P-2→P-2 series column showed a good separation of G1-G8. These results show that the cellooligosaccharides prepared by this method can be used for analytical standards of paper chromatography and filtration.
Journal
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- NIPPON SHOKUHIN KOGYO GAKKAISHI
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NIPPON SHOKUHIN KOGYO GAKKAISHI 26 (12), 538-541, 1979
Japanese Society for Food Science and Technology
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Details 詳細情報について
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- CRID
- 1390001206406583424
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- NII Article ID
- 130003966936
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- NII Book ID
- AN00192587
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- NDL BIB ID
- 2071548
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- ISSN
- 00290394
- http://id.crossref.org/issn/00290394
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed