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Cell fusion between Miura strain and Takahashi strain of Bacillus natto. Proof of fusion by plate-count method.
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- OKADA Noriyuki
- Tropical Agriculture Research Center, Ministry of Agriculture, Forestry and Fisheries
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- NIKKUNI Sayuki
- National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
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- MANABE Masaru
- National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries
Bibliographic Information
- Other Title
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- 納豆菌の細胞融合 コロニーカウントによる融合の証明
- Proof of Fusion by Plate-Count Method
- コロニーカウントによる融合の証明
- Published
- 1991
- DOI
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- 10.3136/nskkk1962.38.79
- Publisher
- Japanese Society for Food Science and Technology
Search this article
Description
Cell fusion was examined using commercial Natto bacteria. An important point is the discrimination of fusants. Following results were obtained; (1) the optimum tonicity for protoplast regeneration (PR) of Bacillus was 0.2M, less than half as the ordinary methods, (2) PR is limited when amino acid-negative mutants were used because of the limitation of casamino acids (CA) content, and (3) an easy plate-count method, namely plating directly in hypertonic media, was possible. Based on above results, nucleic acid-negative mutants (NNM) not affected by CA, and a new protoplast reversion medium containing a low content of sodium succinate, a sufficient amount of CA and a little biotine as well as polyvinylpyrrolidone, were employed. The frequency of back mutation of NNM of Natto bacteria was 10-6 level by the Miura strain with an adenine marker and 10-5 level by the Takahashi strain with an uracil marker. Their PR occured efficiently by the easy plate-count method. The cell fusion between them was shown by giving five proofs in one series of experiments; protoplast formation, its reversion, check of back mutation, control number of fusion and fusant number. The fusion frequency was approximately 0.02%.
Journal
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- NIPPON SHOKUHIN KOGYO GAKKAISHI
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NIPPON SHOKUHIN KOGYO GAKKAISHI 38 (2), 79-85, 1991
Japanese Society for Food Science and Technology
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Details 詳細情報について
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- CRID
- 1390001206406817152
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- NII Article ID
- 130003967841
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- ISSN
- 00290394
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed
