Differential Staining of Collagens Type I, II and III by Sirius Red and Polarization Microscopy

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  • JUNQUEIRA L. C. U.
    Laboratories for Cell Biology, Rheumatology and Experimental Oncology, Medical School, University of São Paulo
  • COSSERMELLI W.
    Laboratories for Cell Biology, Rheumatology and Experimental Oncology, Medical School, University of São Paulo
  • BRENTANI R.
    Laboratories for Cell Biology, Rheumatology and Experimental Oncology, Medical School, University of São Paulo

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  • シリウス赤と偏光顕微鏡によるコラゲンI, II, III型の分別染色
  • Differential Staining of Collagens Type

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Abstract

Organs of fish, amphibian, reptile, bird and mammals when stained by Sirius Red and studied with polarization microscopy present different colors in regions where collagens I, II and III have been described.<br>Collagen type I presented a yellow, orange or red color while collagen type III appeared green. Collagen type II, present in cartilage and chondrosarcoma showed a variable color according to the tissue and the species. Its color and morphology however always permitted its clear distinction from collagens type I and type III.

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