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- 老田 茂
- 農林水産省四国農業試験場
書誌事項
- タイトル別名
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- Extraction and Enzymatic Degradation of Antimicrobial Peptides, .ALPHA. and .BETA.-Thionins, from Barley and Wheat.
- オオムギ コムギ ニ フクマレル アルファ ベータ チオニン コウキン ペプチド ノ チュウシュツ ト コウソ ブンカイ
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説明
Antimicrobial peptides, α and β-thionins, were extracted from barley and wheat flour with 0.075-0.15N HCl. The degradation of α and β-thionins by digestive enzymes at 37°C was examined in vitro. No degradation of α-thionin was observed after the incubation of 10μg of barley α-thionin with 1μg of pepsin A for 16hr. Similar resuls were shown for wheat α-thionin and barley β-thionin. Denaturated wheat α-thionin by S-pyridylethyl modification of cystine residues was degraded by pepsin. Trypsin (0.5μg) and chymotrypsin (5μg) degraded 9.1μg and 7.2μg of wheat α-thionin for 1hr, and produced some partially hydrolyzed peptides. Similar results were obtained for barley α and β-thionins. The partially hydrolyzed peptides had no more antibacterial activity. The partially hydrolyzed peptides were almost completely degraded by leucine aminopeptidase and carboxypeptidase A.
収録刊行物
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- 日本食品科学工学会誌
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日本食品科学工学会誌 47 (6), 424-430, 2000
公益社団法人 日本食品科学工学会
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詳細情報 詳細情報について
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- CRID
- 1390001206407705216
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- NII論文ID
- 10007575610
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- NII書誌ID
- AN10467499
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- COI
- 1:CAS:528:DC%2BD3cXkt1Gmt7Y%3D
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- ISSN
- 18816681
- 1341027X
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- NDL書誌ID
- 5372184
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
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- 抄録ライセンスフラグ
- 使用不可