マカロニの中に巻きこんでラットの大動脈の縦断顕微鏡標本を作る方法

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  • Preparing Vertical Histological Sections of the Rat Aorta Rolled up in a Sheath of Macaroni
  • マカロニ ノ ナカ ニ マキコンデ ラット ノ ダイドウミャク ノ ジュウダン ケンビキョウ ヒョウホン オ ツクル ホウホウ エイブン

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Vertical histological sections of the aorta in rat were easily prepared by supporting it with a piece of macaroni. The procedure is outlined as follows.<br>The aorta of the rat was dissected out, opened longitudinally and fixed in 10% formalin. After fixation, the aorta was cut into a suitable length. Each segment was rolled spirally with the intimal surface inside. It was then put in a cylindrical piece of boiled macaroni, about 4mm in inside diameter and 7mm in length. The block was sectioned at 8-10μ thickness on a freezing microtome. Every section, when put into water, spontaneously separated from the macaroni and stretched into a long longitudinal section. Frozen sections were routinely stained with Sudan III.<br>The remainder of the block was subjected to the dehydration process with ethyl alcohol and embedded in paraffin. At the point of pure alcohol dehydration, the piece of macaroni was removed from the hardened tissue. Paraffin sections were submitted to general staining procedures.

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